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Clinical Hematology

Several artifacts can cause significant and potentially misleading alterations to measured RBC parameters:

  • Old samples cause RBCs to swell, thus increasing PCV and MCV and decreasing MCHC.
  • Lipemia causes a falsely high Hgb reading, and hence a falsely high MCHC.
  • Hemolysis causes PCV to decrease while Hgb remains unchanged, again leading to a falsely high MCHC
  • Underfilling of the tube causes RBCs to shrink, causing PCV and MCV to decrease and MCHC to increase.
  • Autoagglutination causes a falsely low RBC count, and hence a falsely high MCV.


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